ZFIN Image: Xue et al., 2023, Fig. 3

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Figure Caption

Fig. 3

Off-target assessments of hyper ABE and hyper A&C-BEmax.

a Cas9-dependent DNA on and off-target analysis of the indicated targets (HEK site 2 and FANCF site 1) by ABE8e, hyABE, A&C-BEmax, eA&C-BEmax and hyA&C-BEmax in HEK293T cells. Lowercase protospacer sequences represent mismatched bases compared to their corresponding on-target sequences. Data are means � SD (n = 3 independent experiments). b, c Cas9-independent DNA off-target analysis of ABE8e, hyABE, A&C-BEmax, eA&C-BEmax, and hyA&C-BEmax using the modified orthogonal R-loop assay at each R-loop site with nSaCas9-sgRNA. Data are means � SD (n = 3 independent experiments). d, e RNA off-target editing activity by ABE8e, hyABE, A&C-BEmax, eA&C-BEmax and hyA&C-BEmax using RNA-seq. Each biological replicate is listed on the bottom. For a?c, significance was tested by two-tailed Student?s t test. Source data are provided as a Source Data file.

Acknowledgments

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