Figure 7

Effects of AgRP ablation on appetite and growth regulation in the brain of zebrafish. Real-time RT-qPCR analysis was used to measure the mRNA expression levels of (A) orexigenic genes (agrp, agrp2, npy, pdyn, pro-melanin concentrating hormone (pmch), hcrt, gal, and neurotensin (nts)); (B) anorexigenic genes (bdnf, sim1a, trh, tg, avp, nucleobindin 1 (nucb1), corticotropin releasing hormone b (crhb), and oxytocin (oxt)); and (C) GH/IGF axis genes (gh1, ghra, ghrb, igf1, igf2b, igfbp1b, igfbp5b, igfbp6a, and igfbp6b) in MTZ-treated WT and AgRP4.7^NTR larvae at 8 dpf, n = 30. Values are represented as the mean ± S.E.M. *** p < 0.001. (D) WISH experiment revealed the expression levels and patterns of npy, bdnf, trh, tg, and gh1 in the brains of MTZ-treated WT and AgRP4.7^NTR larvae at 5 dpf, n = 30. Scale bars = 100 µm.