ZFIN Image: Liu et al., 2023, Figure 2

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Figure Caption

Figure 2

Generation of tilapia gata2a mutant line by CRISPR/Cas9. (A) Schematic diagram showing gata2a gene structure and sgRNA target site near the translation start site on the antisense strand. (B,C) Generation of the gata2a mutant line. Mutations at the gata2a locus were analyzed by restriction enzyme digestion (B) and DNA sequencing (C). Deletions are indicated by dashes (C). The numbers in brackets represent the bases deleted/inserted from each allele. (D) Identification of F2 genotypes by restriction enzyme digestion assay. Homozygous mutants with different mutation types (+5 bp and −7 bp) were obtained. (E) Sequencing results of the gata2a gene from WT and homozygous mutant fish. (F) Western blot was carried out to show the specificity of the antibody against Gata2a. (G) Immunofluorescence was used to test the specificity of the Gata2a antibody. Scale bar: 50 μm. M, marker; PAM, protospacer adjacent motif.

Acknowledgments

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