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Figure 5
Qualitative food intake assay for MTZ-treated WT and AgRP4.7NTR larvae. (A) Side views of 8 dpf larvae were observed under fluorescence lighting. MTZ-treated WT and AgRP4.7NTR larvae were cultivated with 4-Di-10-ASP-labeled paramecia for 1 h before observing the fluorescent contents in their guts. Scale bar: 2 mm. (B) Relationship between the relative amount of paramecia and fluorescence intensities of ingested paramecia in zebrafish. All values are represented as the mean ± SEM, n = 50. *** p < 0.001.
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