Figure 3

Figure 3

Screening of endocrine disruptors with the Steatogenic Assay on Zebrafish. Zebrafish larvae at 3 days post-fertilization were exposed to the selected compounds at non-toxic concentrations: DDE (A), perfluorinated compounds (B), cadmium (C), butyl-paraben (D), bisphenols (E), phthalates (F) or to vehicle alone, during 48 h. Following exposure, larvae were euthanized and fixed, and steatosis scores (based on lipid fluorescence intensity, lipid droplet density and area in the liver) were calculated based on Nile red staining and image-based automated analysis after confocal microscopy acquisition. Values are mean +/− SEM; with the number of batches and larvae indicated in the table underneath the graph. * p < 0.05, *** p < 0.001 by comparison with control group using Kruskal–Wallis test.