ZFIN Image: Wu et al., 2021, Fig. 3

Image

Figure Caption

Fig. 3

Zebrafish histone H2A interacts with TBK1 and IRF3 to disrupt the formation of TBK1-IRF3 complex. (A) Zebrafish histone H2A interacted with TBK1 and IRF3. (B) Zebrafish histone H2A colocalized with TBK1 and IRF3. (C) Zebrafish histone H2A interacted with TBK1 to inhibit the formation of TBK1-IRF3 complex. For (A, C), co-IP was performed with anti-FLAG-conjugated agarose beads in EPC cells. The cell lysates and bound proteins were analyzed by immunoblotting with the indicated Abs. All experiments were repeated for at least three times with similar results. The expression ratio for IRF3 protein was quantified by Quantity One. For (B), immunofluorescence analysis was performed in EPC cells transfected with H2A-FLAG and TBK1-GFP or IRF3-GFP. Scale bar: 25 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol