Fig. 4
PHLPP1 deficiency suppresses lipid metabolism gene expression
(A and B) Fluorescent images of DyLight?488 OxLDL-treated Hoechst-stained PHLPP1 overexpressing (A) or knockdown (B) RAW 264.7 cells along with quantification (bottom); Each image in (A and B) is a representative image of three different experiments with a minimum of 10 different fields in each experiment. Images are taken at |×20×| magnification; Statistical analysis in (A) was performed using one-way ANOVA followed by Bonferroni?s post hoc test (?p < 0.05, vs Vector-Myr; $p < 0.05 vs PHLPP-Myr); Statistical analysis in (B) was performed using two-tailed t-test (?p < 0.05, vs sh-Luc).
(C and D) BODIPY-labeled cholesterol efflux quantification in PHLPP1 overexpressing (C) or knockdown cells (D); statistical analysis was performed using one-way ANOVA followed by Bonferroni?s post hoc test (?p < 0.05 vs Vector).
(E) Protein levels of foam cell markers in PHLPP1 overexpressing or knockdown cells along with densitometric quantification (bottom). Data are a representative of three independent experiments unless specified. Numerical data are expressed as mean ± SEM [except mean ± SD for (E)-CHOP (n = 2)].