ZFIN Image: Balamurugan et al., 2022, Fig. 2

Image

Figure Caption

Fig. 2

PHLPP1 promotes foam cells in vitro

(A?C) Neutral lipid staining by Oil Red O (A) along with quantification in PHLPP1 overexpressing (A and B) or knockdown RAW 264.7 cells (A and C) with parallel immunoblot analysis (B and C top panels); Images are taken at |�20�| magnification. Each image in (A) is a representative image of four different experiments with a minimum of eight different fields in each experiment. Statistical analysis was performed using one-way ANOVA followed by Bonferroni?s post hoc test (?p < 0.05, ???p < 0.001 vs Vector-OxLDL; ^@p < 0.05 vs PHLPP1-OxLDL; ^$p < 0.05 vs sh-Luc+OxLDL).

(D) Neutral lipid staining in cells overexpressing PHLPP isoforms; Statistical analysis was performed using one-way ANOVA followed by Dunnett?s multiple comparisons test (?p < 0.05 vs Vector).

(E and F) Total cellular cholesterol (E) and FFA levels (F) in RAW 264.7 cells upon PHLPP1 overexpression; Statistical analysis was performed using two-tailed t-test (?p < 0.05, ??p < 0.01 vs Vector). Data are a representative of at least three independent experiments. Numerical data are expressed as mean � SEM.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience