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Fig. 6 Figure 6. CNSL derivatives differentially regulate the expression of PPAR? target genes and adipocyte differentiation in 3T3-L1 cells. 3T3-L1 fibroblasts were differentiated for 11 days in the presence of vehicle (DMSO), 25 ?M of indicated CNSL derivatives or 10 ?M rosiglitazone (Rosi). Cells were harvested for Oil Red O staining and mRNA on day 11. (A) Cells were imaged under 10× magnification (n = 2/per group). (B) Lipid accumulation was quantitated by spectrophotometric analysis of extracted Oil Red O. mRNA expression was analyzed by QPCR for two key regulators of adipogenesis, (C) Ppar? and (D) Cebp?; fatty acid uptake genes (E) aP2 (Fabp4), (F) Lpl, and (G) Cd36; adipose-specific adipokine gene (H) AdipoQ and glucose uptake gene (I) Glut4. Vehicle mRNA expression was set to 1 and Rosi value was set to 100%. Data represent mean ± SD (N = 3). *P < 0.05 vs vehicle and #P < 0.05 vs Rosi; using one-way ANOVA with Holm??idák correction.