ZFIN Image: Albert et al., 2022, Fig. 2

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Figure Caption

Fig. 2

Photoisomerization studies of CS-VIP 8 and interaction with WDR5. (A) Isomerization of CS-VIP 8. (B) UV–vis spectra in aqueous solution after irradiation at 405 and 520 nm reaching the PSS. (C) Reversible cis:trans cycles by alternating illumination at 405 (blue)/520 (green) nm. (D and E) Stability of the cis-isomer at the 520 nm PSS and the trans one at the 405 nm PSS, respectively, at rt in water via HPLC measurements. (F) Results of FP-based assays obtained by generating 405 and 520 nm PSS considering only the residual 10% of the cis isomer present in solution (yellow) and compared with the cis-state as obtained by illumination at 520 nm (green) including calculated K_i.

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