ZFIN Image: Fung et al., 2023, Figure 2

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Figure 2

Pathway enrichment analysis of DEGs identified from RNA-seq data. Differentially expressed genes (DEGs) from RNA-seq data were mapped to the corresponding human orthologs and (A) Gene Ontology (GO) enrichment analysis was performed. The enriched GO annotations under the ‘Biological Process’ category were filtered based on an enrichment threshold >4 with annotations containing DEGs that are either all up- or down-regulated. Filtered annotations were then mapped onto an interaction network as nodes and were connected by an edge if there were common DEGs present between nodes. The thickness of the edge correlates to the number of common DEGs shared between two nodes. The size of each node represents the enrichment score from the GO enrichment analysis. Red indicates the up-regulation of the GO term while blue indicates downregulation. (B) The top 30 results from KEGG enrichment analysis. The histogram shows the direction and average level of dysregulation for all DEGs mapped to each KEGG pathway (left y-axis), and the grey dots indicate enrichment levels (right y-axis).

Acknowledgments

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