ZFIN Image: Guo et al., 2023, Fig. 6

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Figure Caption

Fig. 6

cdkn1a crispant zebrafish larvae show increased bone mineralization.

a Green fluorescent protein (GFP) images of WT and cdkn1a^Cas9/sgRNA-treated zebrafish head regions at 6, 8, and 10 dpf. Scale bars: 500 μm. b Semiquantitative analysis of GFP area and IOD (n = 6). c Alizarin red staining evaluated the effect of cdkn1a^Cas9/sgRNA treatment on bone mineralization at 6, 8, and 10 dpf compared to that in the WT group. Scale bars: 1 mm. d Semiquantitative analysis of Alizarin red staining activity (n = 6). e mRNA expression levels of genes related to osteoblasts (runx2b, sp7, col2a1a, sparc) and osteoclasts (mmp9, mmp13, acp5a, enpp1, sost) in the WT and cdkn1a^Cas9/sgRNA-treated groups (n = 20). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t-test.

Acknowledgments

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