ZFIN Image: Cleghorn et al., 2021, Fig. 2

Image

Figure Caption

Fig. 2

Structural and functional conservation between human and zebrafish IMPDH1.A, negative stain EM of purified human retinal variants IMPDH1(546) & IMPDH1(595) and zebrafish Impdh1a (544) & Impdh1a_tvX1. The scale bar represents 100 nm. B and C, sequence alignment of human retinal variants IMPDH1(546) and IMPDH1(595), zebrafish Impdh1a (544) {"type":"entrez-nucleotide","attrs":{"text":"NM_001002177.1","term_id":"50345007","term_text":"NM_001002177.1"}}NM_001002177.1, and zebrafish retinal variant Impdh1a_tvX1 ({"type":"entrez-nucleotide","attrs":{"text":"XM_005159007.4","term_id":"1207134073","term_text":"XM_005159007.4"}}XM_005159007.4). B, conserved N-terminal alpha-helix in blue and gene beginning in pink. C, residues in orange (ordered) are resolved in cryo-EM structures of the human protein (9), remainder of C-terminus in yellow is flexible and not resolved in structures (9). D, GTP-inhibition curves of zebrafish Impdh1a (544) (solid gray line), Impdh1a_tvX1 (solid black line), and nonassembly Y12A protein (dashed black line). Each data point represents a triplicate and the error bars are standard deviation. impdh, inosine monophosphate dehydrogenase; tv, transcript variant.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.