Fig. 8

Effect of erianin on low oxygen-induced retinal neovascularization in adult zebrafish. (A) Confocal microscopic observation of total retinal vessels in adult zebrafish treated with erianin (200 nM and 1000 nM). (B) Confocal microscopic observation of high vascularity areas of the retina (within the 350 µm square yellow area in panels A–-I) in adult zebrafish treated with erianin (200 nM and 1000 nM). (C) Confocal microscopic observation of low vascularity areas of the retina (within the 350 µm square red area in panels A–I) in adult zebrafish treated with erianin (200 nM and 1000 nM). (I) Fluorescence excitation; (II) Photography by Angio Tool Software. The outline of the vasculature is shown in yellow, the skeleton representation of vasculature in red and branching points are blue; (III) Comparison of vessel branch points, sprouts, and vascular area. * Significant difference compared with normoxia group (paired t-test, P values < 0.05).