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Fig. 6 PLGA-based nanoparticles rapidly associate with AML cells. MOLM-13 cells were incubated Nile Red-loaded nanoparticles at 2% v/v (A). After various times of incubation, cells were washed, and their fluorescence analysed with an Accuri C6 flow cytometer. The results are the average of median fluorescence, and standard deviation from a triplicate experiment. Non-linear regression curves were made using SigmaPlot, ver. 9.01, (Systat Software, Inc., San Jose, CA, USA). (B-K): Cells incubated with Nile Red-loaded nanoparticles were fixed in 2% buffered formaldehyde (in pH 7.4 PBS containing Hoechst 33342) and imaged with confocal microscopy. The scale bars in the confocal micrographs represent 15 µm. See Supplementary Figure S1 for gating strategy