ZFIN Image: Gabellini et al., 2022, Figure 2

Image

Figure Caption

Figure 2 Location of the eight-nucleotide deletion in setd5 gene (setd5-Δ8) and its effects on the expression of selected genes. (a) Schematic representation of the main zebrafish Setd5 protein domains and the corresponding exons, highlighting the SET domain (in green). The 20-nucleotide sequence corresponding to gRNA in exon 7 (in magenta) encoding sequence is indicated, as well as the PAM sequence, aligned to the deleted setd5 mutant sequence obtained by CRISPR/Cas9 gene editing technique. (b–d) Expression of setd5 (b), setd5 paralogues setd2 and mll5 (c), nsd1a and nsd1b isoforms (d) in setd5+/+ and setd5+/− adult zebrafish brains, obtained by RT-qPCR analysis. The values are expressed as 2^−(ΔCt), using bactin1 as the housekeeping gene. (b–d) n = number of adult brains analyzed. Data are expressed as mean ± SEM. Statistical analysis was performed by Student’s t-test. * p < 0.05; ** p < 0.01.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.