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Figure 5 Ipconazole preferentially alters the development of GABAergic neurons. (A) Dorsal views of the brain of the 4-dpf larvae of Tg(pou4f1-hsp70l: GFP)rw0110b and Tg(-8.4neurog1:nRFP)sb3 transgenic fish using confocal microscopy (a–d) and the size analysis of the optic tectum in the embryos treated with ipconazole (e). White box, optic tectum (OT); white arrowhead, neuropil; red arrowhead, periventricular layer, scale bar, 100 μm; (B) mRNA expressions of the following neuronal markers in the 3-dpf embryos using RT-PCR: slc17a6b for excitatory glutamatergic neurons (a), gad1b for GABAergic inhibitory neurons (b), and th for dopaminergic neurons (c); (C) mRNA expressions of the following glial cell markers: olig1 and olig2 for oligodendrocyte formation (a,b) and mbp for myelination (c) in the 3-dpf embryos. * p < 0.05, ** p < 0.01, *** p < 0.001.