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Figure 3 Ipconazole reduces expressions of antioxidant enzymes, caspases, and hsp70. (A) RT-PCR (real-time PCR) shows the antioxidant genes were reduced in the 28-hpf embryos treated with ipconazole at 0, 0.02, 0.2, and 2 μg/mL: gpx1a (a), sod1 (b) sod2 (c), and nrf2 (d); (B) mRNA expression of the apoptotic genes, bcl2 (a), baxa (b), casp9 (c), apaf1 (d), casp3a (e), and casp8 (f), and the necroptotic genes, ripk1 (g) and ripk3 (h), as well as the molecular chaperon hsp70 (i) were monitored. * p < 0.05, ** p < 0.01, *** p < 0.001.
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