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Figure 1 Ipconazole alters larval locomotive behavior in zebrafish development. (A) Survival rate (%) of zebrafish larvae treated with ipconazole, repeated six times (n = 300 each); (B) lateral views of the bright field imaging of the embryos at 25 h post fertilization (hpf) (a–d) and 4 days post fertilization (dpf) (a’–d’), repeated four times (n = 40 each), scale bar, 500 μm; (C) body length of the larvae treated with ipconazole at 4 dpf; (D) lateral views of 3-dpf larvae subjected to polarized light microscopy (a,a’), scale bar, 500 μm; (E) quantitation of the average birefringence of the larval muscle, repeated three times (n = 30 each); (F) locomotor activity of 6-dpf larvae treated with ipconazole. Graphical representation of the average distance traveled in the dark condition. * p < 0.05, ** p < 0.01, *** p < 0.001.