IMAGE

FIGURE 1

ID: ZDB-IMAGE-230110-131
Publication: Scott et al., 2022 - Aerobic glycolysis is important for zebrafish larval wound closure and tail regeneration
All Figures: Figures for Scott et al., 2022

Image

Figure Caption

FIGURE 1

Schematic of the Laconic genetically encoded sensor for lactate. (A) Schematic depiction of the Laconic genetically encoded FRET‐based sensor. A conformational change upon lactate binding to the LldR lactate binding region fused to the Venus chromophore induces a change in energy transfer efficiency from mTFP to Venus (adapted from¹⁷ Figure 1B). (B) Graph depicting emission spectra of Laconic. When bound to lactate, fluorescence intensity detected in the range of mTFP increases and that of Venus decreases due to reduced FRET efficiency, thereby increasing the Laconic ratio as calculated by mTFP/Venus (adapted from¹⁷).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Wound Repair Regen.