Figure 4

A dMpv17 knockdown influences larval NMJ morphology. (A) Synapses in the NMJ on the fourth muscle of third-instar larvae were inspected in elav > GFP-IR, elav > dMpv17-IR⁴²⁶⁷¹, and elav > dMpv17-IR⁵⁶⁸⁸⁹ larvae. The presynaptic terminals were visualized with anti-HRP IgG and the post-synaptic domains with anti-Dlg1 IgG. Scale bar, 10 ?m. (B) Synapse branch length, bouton numbers were quantified. **P < 0.01, *P < 0.05, statistical analysis was performed using Student?s t-test vs elav > GFP-IR. n = 15. (C) The active zone in the NMJ of the fourth muscle was visualized with anti-HRP and anti-Brp IgGs. Scale bar, 10 ?m. (D) The area of Brp signals merged with HRP was quantified. **P < 0.01, statistical analysis was performed using Student?s t-test vs elav > GFP-IR. n = 13.