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Figure 6 Antigen retention of far red tagged keyhole limpet hemocyanin (KLH) by melano-macrophages from immunized goldfish. Goldfish were immunized with KLH and boosted once over a year. These goldfish were then injected with Alexa Fluor 647 tagged KLH and FACS analyzed 13 days later. Green fluorescence is in the GFP channel while far red is done in the APC channel. Background fluorescence was determined using peripheral blood leukocytes. Spleen and Kidney leukocytes contained approximately 3% double positive and <1% single far red positive cells.
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Full text @ Front Immunol