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Figure Caption
Figure 5 Retention of experimentally injected labeled antigen by MMΦs. Leukocytes were isolated from goldfish or zebrafish spleen and kidney 14 days after immunization with BSA-Alexa-647. Red (TRITC) and green (FITC) are MMΦ auto-fluorescence, while BSA-Alexa 647 is seen in the Cy5 channel. Distinct blue spots in the merged image indicate the signal from Alexa-fluor 647. (A, B) Kidney and spleen leukocytes isolated from immunized goldfish. (C) Kidney leukocytes isolated from zebrafish immunized with Alexa-647. Scale bars are 10 µm.
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