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Figure 10 Anti-cancer and anti-metastatic activity of a new HDAC inhibitor 8b against highly metastatic human breast carcinoma MDA-MB-231 cells in zebrafish xenografts. Tg(fli1:EGFP) xenografts (n = 20) were exposed to three different doses of 8b, and analyzed for tumor progression and metastasis after 3-day treatment. Representative fluorescent microscopy images are shown (A); white arrows indicate disseminated cells. The applied treatments markedly reduced the MDA-MB-231 tumor growth (B), number of embryos with metastasis (C), as well as the number of disseminated cancer cells per xenograft (D), as compared to those in the control group (p < 0.001, for all hallmarks). Data are normalized in relation to the control group ((B–D) *** p < 0.001).