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Fig. 5
Figure 5. EphB4a represses track iLV transdifferentiation through suppression of Notch (A–C) The derepressed transdifferentiation of track iLV (Dendra2+DsRed+) in the ephB4a mutant (A, arrow) was rescued by treatment with DAPT, which returned to Dendra2−DsRed+ (B, arrowheads). The statistics show the ratios of transdifferentiation among track iLVs in the ephB4a mutant with or without DAPT treatment (C) (n = 10 larvae; two-tailed unpaired t test; ∗∗∗, p < 0.0001). (D–G) The derepressed transdifferentiation of track iLV (Dendra2+GFP+) in the ephB4a mutant without heat shock (D, arrow) or without dnMAML-Flag (E, arrow) was rescued by the heat-shock-induced, LEC-specific overexpression of dnMAML-Flag, which exhibited Dendra2−GFP+ at 4 dpt (F, arrowhead). The statistics show the ratios of transdifferentiation among track iLVs in the ephB4a mutant groups (G) (n = 10 larvae; two-tailed unpaired t test; ∗∗∗, p < 0.0001). Scale bar, 20 μm. Data are represented as mean ± SD. HS, heat shock. See also Figures S6 and S7.
Reprinted from Developmental Cell, 56(22), Chen, J., Li, X., Ni, R., Chen, Q., Yang, Q., He, J., Luo, L., Acute brain vascular regeneration occurs via lymphatic transdifferentiation, 3115-3127.e6, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell