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Fig. 7
Rapamycin treatment relieves cataract/lens defects in gja8b mutant zebrafish. (A) Statistical analysis of the severity of lens defects in 72 hpf gja8b mutants treated with rapamycin. Horizontal axis labeling shows the final concentration of rapamycin (n > 100 zebrafish for each group). (B) Shows representative images of the nuclei of lens fiber cells in WT, gja8b mutants, gja8b mutants treated with DMSO, and WT and gja8b mutants treated with 25 μM rapamycin at 72 hpf. (C) Shows the quantitative analysis of the average number of lens fiber cell nuclei shown in B (n > 20 zebrafish for each group). (D) Representative images to show the distribution of Kdel, Mip and F-actin in the lens of WT, gja8b mutants, and gja8b mutants treated with 25 μM rapamycin. Scale bars: 50 μm. Mean ± SEM, N.S., p > 0.05, ****p < 0.0001