|
Fig. 7
OP partially rescues D61G Shp2a-induced gastrulation defects and mortality in a dose-dependent manner in zebrafish embryos. Embryos were injected at the one-cell stage with mRNA encoding GFP-2A-Shp2-D61G or GFP-Shp2-wild-type with or without the peptide at concentrations of 0.3, 3, and 5 μM. Non-injected embryos (ni) were evaluated as a control. (A) Ovality of embryos 11 hpf, as indicated by the ratio of the long and short axis. Tukey’s honest significant difference test was performed to assess significance. In the box plot, the horizontal line indicates the median, box limits indicate the 25th and 75th percentiles (interquartile range), and whiskers (error bars) extend to the maximum and minimum values, or to 1.5 times the interquartile range from the 25th and 75th percentiles, if some data points fall outside this range. In the latter case, outliers are indicated as single data points. (B) Embryo lethality. Surviving embryos were counted 1 and 4 days post fertilization. Survival was plotted, and a log rank test was performed to access differences between groups. Nonsignificant (n.s.), p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. The numbers of embryos that were analyzed in each case are indicated in parentheses, in the axis labels (A), or in the legend (B) and ranged from 31 to 61.