IMAGE

Fig. 2

ID
ZDB-IMAGE-221219-2
Source
Figures for Bobone et al., 2021
Image
Figure Caption

Fig. 2

Effect of phosphorylation and sequence length on the binding of IRS-1 pY1172 peptides to the N-SH2 domain. (A) Binding curves for the phosphorylated and unphosphorylated sequence corresponding to the nine-residue region surrounding pY1172 of IRS-1 (see Table 1 for the sequences). The following experimental conditions were used: 1.0 nM CF-P9 (filled symbols and solid lines) and 10 nM CF-P9Y0 (empty symbols and dashed lines). (B) Displacement curves for unlabeled IRS-1 pY1172 analogues of different lengths [P9, P8, and P7 (see Table 1)]. A concentration of labeled peptide equal to 1.0 nM CF-P9, interacting with the N-SH2 domain (40 nM N-SH2), was displaced with increasing amounts of the unlabeled peptides. The bound fraction of labeled peptide is reported as a function of the concentration of the competing, unlabeled peptide. The results of independent, replicate experiments (n = 6 for CF-P9, n = 4 for P9 and P8, and n = 3 for CF-P9Y0 and P7) are reported with different symbols and were fit collectively.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Med. Chem.