ZFIN Image: Ursu et al., 2022, Figure 4

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Figure 4

miR-200c overexpression alters the expression of ZEB1/2 and VEGF-A. (a,b) Cultured human glomerular endothelial cells and podocytes were transfected with an miR-control (miR-Ctrl) or miR-200c mimic (miR-200c), and qPCR was performed. (a) miR-200c-induced downregulation of ZEB1/2 in glomerular endothelial cells. (b) miR-200c-induced downregulation of ZEB1/2 and VEGF-A in podocytes. * p < 0.05, * p < 0.01, n = 4 independent experiments. (c) qPCR showing vegf-aa and vegf-ab mRNA expression after injection of miR-Ctrl or different concentrations of miR-200c mimic in zebrafish. Injections were performed at one two four cell stage. qPCR was performed 120 hpf. * p < 0.05, ** p < 0.01, *** p < 0.001. A pool of 20 zebrafish was used for RNA isolation in each group.

Acknowledgments

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