IMAGE

Figure 4

ID: ZDB-IMAGE-221214-222
Publication: Ursu et al., 2022 - Glomerular Endothelial Cell-Derived miR-200c Impairs Glomerular Homeostasis by Targeting Podocyte VEGF-A
All Figures: Figures for Ursu et al., 2022

Image

Figure Caption

Figure 4

miR-200c overexpression alters the expression of ZEB1/2 and VEGF-A. (a,b) Cultured human glomerular endothelial cells and podocytes were transfected with an miR-control (miR-Ctrl) or miR-200c mimic (miR-200c), and qPCR was performed. (a) miR-200c-induced downregulation of ZEB1/2 in glomerular endothelial cells. (b) miR-200c-induced downregulation of ZEB1/2 and VEGF-A in podocytes. * p < 0.05, * p < 0.01, n = 4 independent experiments. (c) qPCR showing vegf-aa and vegf-ab mRNA expression after injection of miR-Ctrl or different concentrations of miR-200c mimic in zebrafish. Injections were performed at one two four cell stage. qPCR was performed 120 hpf. * p < 0.05, ** p < 0.01, *** p < 0.001. A pool of 20 zebrafish was used for RNA isolation in each group.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.