Figure 1

Figure 1

Dose- and time- response relationships of lateral line hair cell loss following 2 h exposure to cisplatin. (A) Diagram of standardized larval zebrafish cisplatin exposure protocol. Larvae (6 dpf) were placed into cell strainers and moved between cisplatin exposure and EM rinse steps, while being allowed to swim freely in EM during recovery. (B,C) Maximum intensity projection images of neuromast hair cell nuclei labeled with DAPI in either control (B) or cisplatin-treated (C–C”) larvae following 24 h recovery. (D,E) Cisplatin dose response relationship of hair cell loss per neuromast following either 2 h (D) or 24 h (E) recovery. Each dot represents the mean number of hair cells from 47–50 neuromasts/3 experimental trials. Note the delay in maximal hair cell loss at all doses examined. (F) Time response relationship following exposure to 250 µM cisplatin. Each dot represents the mean number of hair cells from 16–20 neuromasts. Maximal hair cell loss appeared to occur between 4–8 h following cisplatin exposure. Bars = SD.