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Figure 4
Experiment on zebrafish. (A) MPDZ crispant zebrafish displayed reduced eye area and body length compared to wildtype zebrafish. The phenotype of the MPDZ-knockdown crispants was more obvious compared to that of the wildtype. (B) Phenotypic ratio indicating the percentage of fish that uniformly showed reduced eye area and body length. The percentage of phenotypic positive fish in the MPDZ gRNA group (n = 71) was significantly greater than that in the WT gRNA group (n = 65) (p < 0.05). (C) Histological examination showing a series of phenotypes, including a small eye and a thin retina.
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