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Figure 3
(A) IF staining showing that MPDZ localization in the ellipsoid zone of the MPDZ crispants retina, with intense green immunostaining (blue arrow), was decreased compared to the wildtype. ZO-1, mainly at the outer limiting membrane (OLM) (yellow arrow), was less expressed following the knockdown of MPDZ. (B) MPDZ knockdown with siRNA in cultured human RPE. IF staining showing the inhibition of MPDZ leading to cytoskeleton rearrangement and reduced ZO-1 expression.
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