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Fig. 5 MOWs? global visualization and analysis. (A) Left panel: representation of the coordinates system and spherical coordinate convention of the double-hemisphere 3D model for the zebrafish embryo. Pv is a general point over the spherical yolk surface, and P?v its projection on the xy plane. Right panel: exemplification of meridians (black) and parallels (blue) on the blastoderm (mb, pb) and yolk sphere (my, py). In both panels, the red circumference represents the blastoderm margin. AP = animal pole, VP = vegetal pole, BM = blastoderm margin. (B) Maximum intensity projection of LSFM imaging of a dclk2-GFP embryo, lateral view, and some of the paths followed to adapt the analysis to the spherical yolk shape. Scale bar 200 µm. (C) Normalized mean intensity kymograph relative to a single meridian of the yolk sphere, over time. Intensity drops (red arrows) reveal MOWs? passage from BM to VP. Speed calculation is performed through slope measurement (elevation angle/time). (D) Some of the paths followed to perform the analysis over the blastoderm hemisphere. Scale bar 200 µm. (E) Normalized mean intensity kymograph relative to a single meridian of the blastoderm hemisphere, over time. Intensity peaks (arrows) reveal the mitotic wave passage from AP to BM. From comparison of (C) and (E) is shown how MOWs follow in time the mitosis but also, in this particular embryo, the presence of an outlier as one mitosis (black arrow in E) is not followed by any MOW. (F) Over the maximum intensity projection of the same embryo (scale bar 200 µm), the analysis along parallels is performed. This produces the normalized mean intensity kymographs relative to blastoderm (G) and yolk (H), showing the wavefronts (dotted lines) of the mitotic waves (G, black dotted line indicates the outlier) and MOWs (H).