|
Fig. 2 LSFM permits to see the microtubule re-arrangement in the whole embryo. (A) dclk2-GFP embryo during synchronous mitosis events of the blastomeres. Lateral view, AP = animal pole, VP = vegetal pole, scale bar 200 µm. (B) Normalized mean intensity profile over time of the blastoderm region as highlighted in (A). The overlaid bars indicate the subsequent developmental stages of the embryo, from 256-cell to sphere stage. (C) The obtained graph for the normalized mean intensity over time on the yolk surface (circular ROI in (A)). Overlaid bars highlight drops of intensity corresponding to MOW passages. (D-F) Three frames (maximum intensity projection) from time-lapse LSFM imaging of the same embryo. The white circumferences indicate the wavefront of the same MOW that is advancing over the eMTN of a dclk2-GFP embryo at cleavage stage, vegetal view. During time, the wavefront travels toward the vegetal pole of the embryo (the travelling is indicated by the reducing size of the overlapped circumference). See also Visualization 1. MC = marginal cell, scale bar 200 µm. Central black region is due to the LS bending caused by the curvature of the sample. (G-I) Zoom in at the square region highlighted in (D), visualizing the eMTN (G) before (diffuse network), (H) during (sparser MTs), and (I) shortly after (re-diffused network) the MOW passage. Scalebars 50 µm.