ZFIN Image: Nelson et al., 2022, Figure 1.

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Figure Caption

Figure 1.

Reporter fluorescence of zebrafish larvae. A, Larvae from outcrosses of crx:mYFP-2A-tr?2 heterozygotes are either heterozygous or wild-type controls (WT). The heterozygotes are recognized by both pupil (p) and heart (h) fluorescence. The yolk sac (y) is autofluorescent. B, The cornea of a 5-d transgenic eye isolated from a crx:mYFP-2A-tr?2 larva is penetrated with a patch electrode for ERG recordings. The eye is <0.5-mm diameter. C, The gnat2:mYFP-2A-tr?2;mpv17?/? gain-of-function phenotype is studied on a roy orbison (mpv17?/?) background strain. The darkly pigmented, nonreflective iris of this control strain aids in visualizing the dim transgenic fluorescence of the pupil (p). The yoke (y) is autofluorescent. D?F, Live confocal imaging of retinas in 6-dpf larvae. D, WT red (red) and UV (green) cone morphology visualized with tr?2:tdTomato and sws1:GFP fluorescent reporter transgenes. E, The mYFP construct in the crx:mYFP-2A-tr?2 transgene causes cones (C) and bipolar cells (BC) to fluoresce. F, The mYFP construct in the transgene gnat2:mYFP-2A-tr?2 marks only cone cells.

Acknowledgments

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