Fig. 6

Fig. 6 ROS activity and apoptosis in C6 glioma cells after incubation with CUR, CUR-ZpD NPs and CUR-ZpD-G23 NPs (Curcumin = 5 μg ml⁻¹) for 4 h. (A) Fluorescence microscopy images of oxidized H₂DCFDA (DCF), i.e., a measure of ROS activity, in C6 glioma cells following treatment with CUR, CUR-ZpD and CUR-ZpD-G23 NPs. (B) Quantification of mean DCF fluorescence intensity of cells following treatment with CUR, CUR-ZpD and CUR-ZpD-G23 NPs. (C) Apoptosis values in C6 glioma cells as induced by incubation with CUR, CUR-ZpD NPs and CUR-ZpD-G23 NPs for 24 h, obtained by Annexin V-FITC/PI detection by flow cytometry. (D) Representative Annexin-V/PI flow cytometry dot plots. Top right quadrant shows Annexin-V/PI double positive cells. (*Significantly different from control; ^# significantly different from CUR at equivalent CUR concentrations, n = 3 in triplicate, p < 0.05).