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Fig. 3

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ZDB-IMAGE-221208-3
Source
Figures for Zhang et al., 2021
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Figure Caption

Fig. 3 Cellular uptake and tumor spheroid penetration of ZpD and ZpD-G23 NPs. (A) CLSM images of C6 glioma cells after incubation with coumarin-6, C6-ZpD NPs and C6-ZpD-G23 NPs for 4 h. Green colors represent coumarin-6 fluorescence. Cell nuclei were stained with DAPI (blue). Scale bars represent 20 μm. (B) The mean fluorescence intensity of cells with different treatment (**Significantly different from coumarin-6, p < 0.005). (C) CLSM images for 3D-cultured C6 glioma tumor spheroids after treatment with coumarin-6, C6-ZpD NPs and C6-ZpD-G23 NPs for 4 h (scale bar represents 100 μm). Confocal sections at 10, 20, 30 and 40 μm from the rim of a spheroid are shown. (D) Semi-quantitative analysis (% penetration area and mean fluorescence intensity) of CLSM images taken at 40 μm from the top of spheroids using ImageJ software. Data represented as mean ± S.D. of three independent experiments and 12 spheroids per condition (*p < 0.05, **p < 0.005).

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