Fig. 8

Fig. 8 Fig. 8. Trunk neural phenotype in cont BMP-treated (Z-stack). (A,D) A mock animals showing wild-type phenotype. (B,E) 4q+22 ​h continuous animals showing a severe VNC collapse phenotype. (C,F) 4q+22 ​h continuous animals showing a mild VNC collapse. (A–C) acetylated-tubulin showing neurotroch cilia, (A′-C′) acetylated-tubulin in the VNC. (D–F) Hoechst nuclear stain. (D′-F′) 5HT (D″-F″) Pax, arrowhead: normally bilaterally-symmetric cells on either side of the midline in each segment. Figure panels with the same letter represent the same animal. A, anterior, L, left side, nt, neurotroch; pt, prototroch; tt, telotroch; VNC, ventral nerve cord; scale bar ​= ​50 ​μm (G) Boxplot showing the significant effect of treatment on proportion of segments with collapsed hemiganglia, letters indicate significance groups.