ZFIN Image: Cudia et al., 2021, Fig. 7

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Fig. 7

NMR and SEC-MALS of GCAP5 dimerization site mutants. (A) ¹H?¹⁵N HSQC spectra of ¹⁵N-labeled GCAP5^M25E (red) and GCAP5^WT (black). (B) ¹H?¹⁵N HSQC spectra of ¹⁵N-labeled GCAP5^H18E/Y21E (red) and GCAP5^WT (black). (C) Ion exchange chromatogram to isolate the monomeric fraction (marked by an asterisk) of GCAP5^H18E/Y21E. (D) Size exclusion chromatogram of GCAP5^H18E/Y21E. (E) MALS Zimm plot analysis of the monomeric fraction of GCAP5^H18E/Y21E that determined a molar mass of 24 � 2 kDa. (F) Ion exchange chromatogram to isolate a monomeric fraction (marked by an asterisk) of GCAP5^R22D. (G) Size exclusion chromatogram of GCAP5^R22D. (H) MALS Zimm plot analysis of GCAP5^R22D that determined a molar mass of 29 � 4 kDa. The experimental conditions for MALS are given in Materials and Methods.

Acknowledgments

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