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Fig. 6
Activation of photoreceptor guanylate cyclase by GCAP5 mutants. Purified myristoylated GCAP5 wild type (WT), Cys-less (5A), and variants (H18A/Y21A, H18E/Y21E, R22A, R22D, M25E, N56C, N139C, and S159C) were incubated with HEK293 cell membrane suspensions containing photoreceptor human guanylate cyclase (GC-E). The concentrations of wild type GCAP5 and variants were each 3 ?M, and the free Ca2+ concentration was adjusted to 30 ?M (dark gray bar) or 10 nM (light gray bar) using a Ca2+/EGTA buffer system.
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