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Fig. 1
Amino acid sequence alignment and secondary structure of GCAP1 and GCAP5. EF-hand motifs are shaded in color (green for EF1, red for EF2, cyan for EF3, and yellow for EF4). Nonconserved cysteine residues (C15 and C17) in GCAP5 are highlighted in red. Single cysteine residues (C15, C17, T26C, C28, N56C, C69, C105, N139C, E152C, and S158C) modified by nitroxide for DEER studies are highlighted in blue. ?-Helices and ?-strands are depicted by cylinders and arrows, respectively. Swiss Protein Database accession numbers are Q90WX4 (bovine GCAP1) and Q5MAC8 (zebrafish GCAP5).
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