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Figure 7. OMS-induced mtROS production mediates inflammatory signalling and antimicrobial responses during Mabc infection. (a) Representative immunofluorescence images (scale bar = 20 μm) and quantitative analysis of relative fluorescence intensities of mtROS. BMDMs were infected with Mabc (MOI of 3), with or without OMS treatment (5 μM) for 2 h. (b – d) qPCR analysis of Tnf, Il1b, and Il6 mRNA expression in BMDMs. BMDMs were infected with Mabc (MOI of 3), and treated with OMS (5 μM) or MitoTEMPO (50 μM) for 6 h. (e) BMDMs were infected with Mabc (MOI of 1), and treated with OMS (5 μM), MitoTEMPO (200 μM), and L-NMMA (1 mM) for 2 days. Intracellular survival was assessed by CFU analysis. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparison test (a right, b–e). Data are means ± SD or SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. **** p < 0.0001 BMDMs, bone marrow derived macrophages, OMS, Ohmyungsamycin A; a.u., arbitrary unit; n.s., not significant.