ZFIN Image: Edirisinghe et al., 2022, Figure 3

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Figure 3

Histological analysis of zebrafish eye and corneal re-epithelialization following the corneal injury and PDRN treatment. (A) Representative image of H&E stained zebrafish eye, cornea, and graphical illustration of the corneal epithelium. (B) Representative photograph of the whole eye of un-injured (negative control), cornea-injured vehicle, and cornea-injured PDRN-treated groups (scale bars, 40 μm), and (C) photographs of selected central epithelium surface of the cornea (scale bars, 200 μm.) at 1 and 72 hpi. Regenerated epithelial cell layers were marked with black arrows. Quantification of corneal regeneration based on (D) epithelium thickness, and (E) number of epithelium cell layers at 72 hpi. One-way ANOVA was performed to determine the statistical significance; * p < 0.05, ** p < 0.01, *** p < 0.001 (n = 8).

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Acknowledgments

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