FIGURE 3

FIGURE 3

Chondrosarcoma cells carrying COG4^p.G516R mutation show different features compared to WT and COG4-KO cells. (A) Representative images of aggregates formed by WT or COG4^p.G516R cells in pellet culture. Bar, 1 mm. (B) Diameter measurement of the spheroids on Day 10 (n = 5). Data were presented as scatter dot plot with mean and SEM. (C) Diameter measurement of the spheroids over 9 days (n = 5). Data were presented as mean with SD. (D) Representative images of H&E staining of spheroids on Day 6. Bar, 300 µm. (E) Representative images of TUNEL assay on Day 4. Bar, 50 µm. (F) Statistical analysis of (E) (n = 7) Data were presented as mean with SD. All experiments were performed in duplicate with similar results. Unpaired two-tailed t-test was used. ****, p < 0.001; **, p < 0.01.