Figure 5

Figure 5

Antioxidant effect of PEs in vivo. Thirty zebrafish embryos were incubated either with PEs at different concentrations or Tripeptide-1 as the positive control (PC) for 24 h. After, toxicity in zebrafish embryos was determined through bright-field microscopy while the ROS levels were detected through a fluorescent dye H2DCFDA. Briefly, 20 μg/mL of H2DCFDA replaced the PEs to incubate with embryos for 1 h in the dark. After washing three times, the fluorescence was observed via fluorescence microscopy (c) and the fluorescence intensity was obtained using a microplate reader (b). ^∗∗p < 0.01, ^∗∗∗p < 0.001 compared to the nontreatment group.