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Figure 5

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Publication: He et al., 2022 - Anti-Inflammatory and Antioxidant Properties of Physalis alkekengi L. Extracts In Vitro and In Vivo: Potential Application for Skin Care
All Figures: Figures for He et al., 2022

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Figure 5

Antioxidant effect of PEs in vivo. Thirty zebrafish embryos were incubated either with PEs at different concentrations or Tripeptide-1 as the positive control (PC) for 24 h. After, toxicity in zebrafish embryos was determined through bright-field microscopy while the ROS levels were detected through a fluorescent dye H2DCFDA. Briefly, 20 μg/mL of H2DCFDA replaced the PEs to incubate with embryos for 1 h in the dark. After washing three times, the fluorescence was observed via fluorescence microscopy (c) and the fluorescence intensity was obtained using a microplate reader (b). ^∗^∗p < 0.01, ^∗^∗∗p < 0.001 compared to the nontreatment group.

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