ZFIN Image: He et al., 2022, Figure 4

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Figure 4

Antisenescent effect of PEs on HFF-1. Cells were treated with 20 mg/mL of D-gal together with 100 ng/mL of PEs for 72 h. After, cells were stained according to the manufacturer's protocol. β-gal positive cells (in blue) were observed through microscopy (scale bar represents 20 µm) (a) and counted (b), moreover, the anti-inflammatory effect of PEs on HFF-1 was evaluated according to the ELISA experiment of IL-1β in cell culture medium after treatment with 400 µM of H₂O₂ with/without 100 ng/mL PEs (c). Each experiment was performed in triplicate. ^∗^∗p < 0.01 compared to the groups of D-gal- or H₂O₂-treatment.

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