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Fig. 3
The effect of absent blood flow on trunk vessel development. (A, B, C and D) Trunk vasculature from 2 to 5dpf in Tg(kdrl:HRAS-mCherry)s916 uninjected control. (E, F, G and H) Trunk vasculature in control MO (n = 7–10; two experimental repeats). (I, J, K and L) Trunk vasculature in tnnt2a MO. Topology of the trunk vasculature is established in the absence of flow, but morphology of the CV is severely altered (black arrowheads) and intussusceptions are lacking (green arrowhead; d – dorsal, v – ventral; representative images colour-coded by depth).
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