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Figure 4
Exogenous expression of GmIFN–γ. (A) Optimization of prokaryotic expression conditions. Nos. 1–5 indicate that the protein was induced for 1–5 h. No. 6 was set as the control. Target protein was marked by arrow. (B) Purification of GmIFN–γ from E. coli. Target protein was marked by arrow. (C) GmIFN–γ expressed in yeast GS115 and detected using Western blot at 24, 72 and 96 h, respectively.
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