scRNA-seq analysis identified 10 cell types in the WT intestine at 5 dpf. (A) Visualization of integrated unsupervised clustering of cells in the UMAP plot of the sequenced 1229 cells from the WT intestines (left), together with cell annotations (right). (B) Dot plot of 4 marker genes for each cell cluster (cluster 0–cluster 9) for cell type annotation. (C,D) Feature plot together with WISH showing the expression of feature genes in the goblet (agr2), enteroendocrine (pyyb) and smooth muscle cells (tagln) (C), and in the intestinal bulb (gsto2), posterior bulb plus mid-intestine (fabp2, fabp6) and hindgut (ctsbb) cells (D).
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