Figure 1

Figure 1

Identification of DEGs between the 5 dpf larval intestine and liver by RNA-seq analysis. (A) Flow chart showing the micro-dissection of the intestinal tube and liver bud from the zebrafish embryos at 5 dpf. The intestinal tube and liver bud are outlined with white and yellow dashed lines, respectively, in the embryo. (B) Heatmap showing the hierarchical clustering of the 2110 intestine-enriched and 1637 liver-enriched DEGs in the three intestine and three liver RNA-seq samples. (C) Heatmap showing the hierarchical clustering of the 385 intestine-specific and 308 liver-specific genes. (D,E) WISH showing the expression of 8 intestine-specific genes (D) and 8 liver-specific genes (E). Note that the ifi30 transcripts were enriched in the hindgut. The abbreviations of gene names followed the ENSEMBL naming system.